ABSTRACT:- Plants usually propagated by seed can now be cultured In vitro yield thousands of identical plants a number of agronomically important medicinal plants. Specific characteristics such as disease and herbicide resistance also can be selected for while plants are in culture concentration of cytokinine Induction M. venkateshwarlu (2021).  The MS medium supplemented with different concentrations of Cytokinins alone and in combinations with various auxins were used for high frequency shoot regeneration from Leaf explants. In vitro shoot and multiple shoot induction was achieved in one of the important medicinal plants of Cocculus orbiculatus which has been historically been used to treat a wide assortment of diseases. MS medium supplemented with 1.0 mg/l BAP was found to be optimum to induce shoots (100%) directly from the Leaf explants.  Significant increase in the number of shoots per explants was found in MS medium supplemented with 1.0 mg/l BAP and 15 mg/l Adenine Sulphate. Desirable plants are cloned through tissue culture to produce genetically identical plants in a process called   In vitro clonal propagation. The large variety of medicinal and crop plants in both laboratories and plant nurseries. All the tested combinations have little effect on increasing the number of shoots.  The present study established reliable and reproducible protocol for rapid multiple shoot induction from Leaf explants of Cocculus orbiculatus using different concentrations and combinations of cytokinins. Micro shoots developed in the above culture rooting was also induced by BAP, NAA+Kn 0.5mg/l to 5.0 mg/l from callus. Callus tissue has the ability to plant or plant organ in a process called dedifferentiation in plant tissue culture plant growth is usually initiated from leaf explants of Cocculus.

Keywords: Propagation, Cocculus orbiculatus, BAP, Kn, In Vitro cultivation.